Enterocoocci are ubiquitous in nature and being formidable nosocomial pathogen it acquires high level resistance to multiple antibiotics either by mutation or by receipt of foreign genetic material through the transfer of plasmids and transposons. With rising prevalence of High-level aminoglycoside resistance (HLAR) and Vancomycin resistance enterococci (VRE) regularly screening can limits the spread of multidrug resistant infection. The present study was carried out at Fortis Escorts Hospital from various clinical samples, and urine, blood and pus specimen was found to be highly infected. Phenotypic screening was determined via Kirby-Bauer disk diffusion method on Mueller Hinton agar or Agar screen method following clinical laboratory standards. Among 4458 samples, a total of 59 enterococcal species were isolated which include 49% E.faecium, 42% E.faecalis, 7% E.gallinarum and 2% E.avium. Maximum 20.3% of enterococcal isolates were observed in patient of age group 51-60 years and more in females than males. Total HLAR among E.faecium isolates (59%) was higher than E.faecalis (28%). Vancomycin resistance of 20.3% was observed among the Enterococcus species. E.faecium was most commonly isolated from hospitalized patients and E.faecalis was most commonly isolated from outpatients. Linezolid was found the most sensitive antibiotic against the Enterococcus strain followed by Nitrofurantoin (88.8% sensitivity) and Teicoplan (79.6%). Combinedly Ampicillin, Vancomycin and HLA (high level aminoglycoside) noted in 28% of Enterococcs strain.