Profenofos, an organophosphate pesticide, is known for its deleterious effects on the male reproductive system, primarily through oxidative stress and endocrine disruption. This study aimed to evaluate both the biochemical and reproductive toxicity induced by profenofos and the potential for spontaneous recovery in male rats without therapeutic intervention. Adult male Wistar rats were administered profenofos (25 & 50 mg/kg body weight) orally for 45 days, followed by a 45-day recovery period without treatment. Profenofos exposure significantly reduced serum testosterone levels, total protein, and antioxidant enzymes (SOD and GSH), while increasing lipid peroxidation (LPO). Sperm count, motility, and viability were also markedly impaired. After 45 days of withdrawal, partial but significant recovery was observed in most biochemical parameters, including a non-significant recovery of antioxidant enzyme levels. Testosterone levels and sperm quality showed non-significantly improvement, indicating the capacity of the reproductive system for natural recovery over time. However, full restoration to baseline values was not achieved. These findings suggest that while profenofos induces significant reproductive toxicity, endogenous repair mechanisms can mediate partial recovery in the absence of treatment, highlighting the resilience and limitations of natural physiological repair processes.